Use of in situ hybridization to study human megakaryocytopoesis in vivo.
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Use of in situ hybridization to study human megakaryocytopoesis in vivo. by Linda Jane Hainey

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Published by University of Manchester in Manchester .
Written in English


Book details:

Edition Notes

Thesis (M.Sc.), University of Manchester, Faculty of Medicine.

ContributionsUniversity of Manchester.
The Physical Object
Pagination233p.
Number of Pages233
ID Numbers
Open LibraryOL21068992M

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In situ hybridization detection of light chain mRNA in routine bone marrow trephines from patients with suspected myeloma. Br J Haematol. Nov; 73 (3)– Walker RA, Senior PV, Jones JL, Critchley DR, Varley JM. An immunohistochemical and in situ hybridization study of c-myc and c-erbB-2 expression in primary human breast by: Abstract. In situ hybridization has a number of features which make it particularly suitable for use in studies of viral disease. Not only can it confirm the presence of specific viral DNA or RNA sequences in a range of histological preparations, but by demonstrating the precise tissue, cellular and subcellular location of the virus it can correlate the presence of a virus with its Cited by: 2. The explosion of interest in specific molecules important for brain function and dysfunction has drawn individuals from diverse backgrounds toward the use of in situ hybridization techniques. Study of the brain demands the anatomic precision and biochemical specificity that this approach can potentially bring. In situ hybridization is now recognized as an important technique in many areas of molecular biological research and its associated cli- cal studies. There is very little doubt that the applications of this te- nique will multiply in coming years since older methods are rapidly superseded by newer ones that enable the investigation of even more.

Eastmond DA, Schuler M, Rupa DS. Advantages and limitations of using fluorescence in situ hybridization for the detection of aneuploidy in interphase human cells. Mutat Res. ; – doi: /(95) Levsky JM, Singer RH. Fluorescence in situ hybridization: past, present and future. M.A. Hayat, in Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas, In situ Hybridization. In situ hybridization (ISH) is one of the basic methods of developmental biology and provides the advantage of visualizing and even quantifying clinically relevant molecules in a morphological context. It is one of the most important techniques to visualize gene.   In these new assays, manual protocols will be replaced by automation and fluorescence will be replaced by permanent signals visible with standard light microscopy. These brightfield ISH methods include chromogenic in situ hybridization (CISH; Lambros et al., ) and silver in situ hybridization (SISH; Tubbs et al., ). Most publishers and style guides instruct authors not to use italics for such phrases. Both Springer and Elsevier, for example, insist on setting "in vitro," "in vivo," and "in situ" in normal, or Roman, font, and so does the Chicago Manual of Style and Scientific Style and Format.

Abstruct. In situ hybridization (ISH) is an important method for determining the distribution of mRNA within cells or tissue preparations by hybridization of a nucleic acid probe (either DNA or RNA) with a specific target nucleic acid (usually mRNA) (1, 2).Thus, ISH enables the localization of transcripts within cells, tissues, and whole body and allows a neuroanatomic comparison of specific. Translational Relevance. To better understand microRNA (miRNA) function in carcinogenesis, sensitive and reproducible methods for in situ hybridization (ISH) are needed. For experimental analysis, we have chosen analysis of miR expression during the process of colorectal cancer development, and strictly determined conditions for specific detection of miR using several human cell lines. about in situ hybridization. In situ hybridization is the technique by which a labeled, single-stranded RNA or DNA molecule in solution is hybridized to immobilized single-stranded RNA or . In situ hybridization is a powerful technique for identifying specific mRNA species within individual cells in tissue sections, providing insights into physiological processes and disease pathogenesis.